防御素α5(DEFa5)重組蛋白
[ PROPERTIES ] Source: Prokaryotic expression. Host: E. coli Residues: Glu20~Arg94 Tags: N-terminal His-Tag Tissue Specificity: Intestine. Subcellular Location: Secreted. Cytoplasmic vesicle, secretory vesicle. Purity: >92% Traits: Freeze-dried powder Buffer formulation: PBS, pH7.4, containing 1mM DTT, 5% trehalose, 0.01% sarcosyl and Proclin300. Original Concentration: 200ug/mL Applications: SDS-PAGE; WB; ELISA; IP; CoIP; Purification; Amine Reactive Labeling. (May be suitable for use in other assays to be determined by the end user.) Predicted isoelectric point: 5.9 Predicted Molecular Mass: 14.8kDa Accurate Molecular Mass: 15kDa as determined by SDS-PAGE reducing conditions. [ USAGE ] Reconstitute in PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
[ STORAGE AND STABILITY ] Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 oC for one month. Aliquot and store at -80 oC for 12 months. Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 oC for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
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