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NC-37細胞

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產品名稱: NC-37細胞
產品型號: NC-37
產品展商: HZbscience
產品文檔: 無相關文檔

簡單介紹

NC-37細胞應如何避免細胞污染,細胞污染的種類可分成**、酵母菌、霉菌、病毒和霉漿菌。主要的污染原因為無菌操作技術不當、操作室環境不佳、污染之血清和污染之細胞等。嚴格之無菌操作技術、清潔的環境、與品質良好之細胞來源和培養基配制是減低污染之*好方法。NC-37細胞何時須更換培養基?視細胞生長密度而定,或遵照細胞株基本數據上之更換時間,按時更換培養基即可。


NC-37細胞  的詳細介紹

NC-37細胞

ATCC Number: CCL-214?

相關**: **瘤

細胞類型: B**細胞

細胞形態: **樣

是否是腫瘤細胞: 1

物種來源: 人

運輸方式: 凍存運輸

數量: 大量

生長狀態: 懸浮生長

器官來源: 外周血

Designations: NC-37

NC-37細胞Depositors: J Wolff

Biosafety Level: 2 [Cells Contain HERPESVIRUS ]

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Homo sapiens

Morphology: lymphoblast


Source: Organ: peripheral blood

Disease: Burkitt's lymphoma

Cell Type: B lymphoblast;

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. NC-37細胞Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Isolation: Maywood New Jersey, United States

Isolation date: February, 1967

DNA Profile (STR): Amelogenin: X,Y

CSF1PO: 10,12

D13S317: 13,14

D16S539: 8,11

D5S818: 10,13

D7S820: 10

THO1: 6,7

TPOX: 8,13

vWA: 16

Cytogenetic Analysis: modal number = 48; range = 40 to 51

Isoenzymes: G6PD, B

Gender: male

Comments: NC-37細胞The NC-37 line has been utilized as a target cell for EBV superinfection studies and has proven useful in chemical induction studies of the latent EBV genome and in the propagation of various (retroviruses) oncornaviruses. The consistency of the line's characteristics has made it a useful tool in a wide variety of biochemical, immunological, virological and cell biological studies.

Each cell contains an average of 60 EBV genome copies. The cells are EBNA positive (EBNA+) and surface immunoglobulin negative (sIg-).

Although deposited with the ATCC as a lymphoblastoid cell line established from a 34 year old male caucasian, DNA fingerprinting has shown this line to be a derivative of Raji (see ATCC CCL-86), a Burkitt's lymphoma cell line.

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Temperature: 37.0°C

Subculturing: Protocol: Cultures can be maintained by addition or replacement of fresh medium. Maintain cultures at 5 X 10(5) viable cells/ml. A maximum density of upto 2 X 10(6) is possible.

Medium Renewal: Add fresh medium (20% to 30% by volume) every 3 to 4 days

Preservation: Complete growth medium supplemented with 5% (v/v) DMSO.

Related Products: derivative:ATCC CCL-86

References: 22512: Durr FE, et al. Studies on the infectivity and cytopathology of Epstein-Barr virus in human lymphoblastoid cells. Int. J. Cancer 6: 436-449, 1970. PubMed: 4321017

26048: Mayyasi SA, et al. NC-37細胞The coating reaction of the herpes-type virus isolated from malignant tissues with an antibody present in sera. Cancer Res. 27: 2020-2024, 1967. PubMed: 6073499

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