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29SR細胞

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產品名稱: 29SR細胞
產品型號: 29SR
產品展商: HZbscience
產品文檔: 無相關文檔

簡單介紹

29SR細胞應如何避免細胞污染,細胞污染的種類可分成**、酵母菌、霉菌、病毒和霉漿菌。主要的污染原因為無菌操作技術不當、操作室環境不佳、污染之血清和污染之細胞等。嚴格之無菌操作技術、清潔的環境、與品質良好之細胞來源和培養基配制是減低污染之*好方法。29SR細胞何時須更換培養基?視細胞生長密度而定,或遵照細胞株基本數據上之更換時間,按時更換培養基即可。


29SR細胞  的詳細介紹

29SR細胞

生長狀態: 懸浮生長

ATCC Number: CRL-2262?

相關**: 其他**

年限: 11 years

數量: 大量

運輸方式: 凍存運輸

細胞形態: **樣

細胞類型: **母細胞

是否是腫瘤細胞: 1

物種來源: 人

Designations: SR

Depositors: M Beckwith

29SR細胞Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Homo sapiens

Morphology: lymphoblast


Source: Disease: large cell immunoblastic lymphoma

Derived from metastatic site: pleural effusion

Cell Type: lymphoblast

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Antigen Expression: Hle-1 +; HLA DQ +; HLA DR +; CD25 +; CD19 -; CD20 -; CD21 -; CD22 -; T cell receptor (TCR) -

DNA Profile (STR): 29SR細胞Amelogenin: X,Y

CSF1PO: 14,15

D13S317: 11

D16S539: 11,13

D5S818: 11

D7S820: 9,12

THO1: 6,7

TPOX: 8,11

vWA: 17,18

Age: 11 years

Gender: male

Ethnicity: Caucasian

Comments: SR is a human lymphoma cell line originated in 1983 by Walter J. Urba and Dan L. Longo.

29SR細胞SR is of undetermined cellular origin because it expresses no markers unique to B, T, natural killer (NK) or monocyte-lineages.

Exposure of SR cells to protein kinase C activating phorbol esters such as PMA and PdBu do not induce growth inhibition.

SR cells have been reported to be Epstein-Barr virus genome negative.

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Subculturing: Medium Renewal: Every 2 to 3 days

Cultures can be maintained by addition or replacement of medium. Establish new cultures at 2 X 10 exp5 viable cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6.

Preservation: Culture medium, 95%; DMSO, 5%

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001

recommended serum:ATCC 30-2020

References: 22555: Beckwith M, et al. 29SR細胞Phorbol ester-induced, cell-cycle-specific, growth inhibition of human B-lymphoma cell lines. J. Natl. Cancer Inst. 82: 501-509, 1990. PubMed: 2313723

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