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SU.86.86細胞

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產品名稱: SU.86.86細胞
產品型號: SU.86.86
產品展商: HZbscience
產品文檔: 無相關文檔

簡單介紹

SU.86.86細胞應如何避免細胞污染,細胞污染的種類可分成**、酵母菌、霉菌、病毒和霉漿菌。主要的污染原因為無菌操作技術不當、操作室環境不佳、污染之血清和污染之細胞等。嚴格之無菌操作技術、清潔的環境、與品質良好之細胞來源和培養基配制是減低污染之*好方法。SU.86.86細胞何時須更換培養基?視細胞生長密度而定,或遵照細胞株基本數據上之更換時間,按時更換培養基即可。


SU.86.86細胞  的詳細介紹

SU.86.86細胞

年限: 57 years

數量: 大量

運輸方式: 凍存運輸

ATCC Number: CRL-1837?

器官來源: 胰腺

相關**: 導管癌

生長狀態: 貼壁生長

是否是腫瘤細胞: 1

物種來源: 人

細胞形態: 上皮樣

Designations: SU.86.86

SU.86.86細胞Depositors: WD Holder

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Homo sapiens

Morphology: epithelial


Source: Organ: pancreas

Disease: ductal carcinoma

Derived from metastatic site: liver

Cellular Products: carcinoembryonic antigen CEA)

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Tumorigenic: Yes

DNA Profile (STR): SU.86.86細胞Amelogenin: X

CSF1PO: 10,11

D13S317: 11,12

D16S539: 11,13

D5S818: 7,13

D7S820: 8,11

THO1: 9.3

TPOX: 8

vWA: 17

Age: 57 years

Gender: female

Ethnicity: Caucasian

Comments: The cells can be lysed completely in vitro by autologous and allogeneic lymphokine activated killer (LAK) cells in the presence of interleukin 2 (IL-2). They are relatively insensitive to autologous and allogeneic natural killer (NK) cell lysis.

Propagation: SU.86.86細胞ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Subculturing: Protocol: Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes. 1. Remove and discard culture medium.2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin-0.53 mM EDTA solution to remove all traces of serum, which contains trypsin inhibitor.3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and place at 37?C to facilitate dispersal. Observe cells within 5 to 10 minutes under an inverted microscope 4. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting. 5. To remove trypsin-EDTA solution, transfer cell suspension to a centrifuge tube and spin at approximately 125 xg for 5 to10 minutes.6. Discard supernatant and resuspend cells in fresh growth medium. Add appropriate aliquots of cell suspension to new culture vessels.Recommended seeding densities of 5 X 10(3) to 4 X 10(4) viable cells/cm27. Place culture vessels in incubators at 37?C.Note: subcultures can also be prepared by scraping the cells from the floor of the flask. Add fresh medium, aspirate the scraped cells to disperse them and dispense into new flasks.

Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended by depositor

Medium Renewal: 2 to 3 times per week

Preservation: Freeze medium: Culture medium ,40% ; DMSO,10% ; FBS, 50% FBS..

Storage temperature: liquid nitrogen vapor phase

References: 2159: Drucker BJ, et al. A new human pancreatic carcinoma cell line produced for adoptive immunotherapy studies with lymphokine-activated killer cells in nude mice. SU.86.86細胞In Vitro Cell. Dev. Biol. 24: 1179-1187, 1988. PubMed: 3264833

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